TY - JOUR
T1 - Tagging of biomolecules with deuterated water (D2O) in commercially important microalgae
AU - Saha, Sushanta Kumar
AU - Hayes, Jeremiah
AU - Moane, Siobhan
AU - Murray, Patrick
PY - 2013/7
Y1 - 2013/7
N2 - To understand the effect of any biomolecules in specific metabolic pathways in humans, bioavailability and for other basic understanding, stable isotopically-labelled biomolecules (preferably deuterated) is the fundamental pre-requisite. Production of deuterated biomolecules such as, astaxanthin, β-carotene, lutein, chlorophyll-a, and eicosapentaenoic acid (EPA, 20:5n-3) by metabolic tagging have been shown in commercially important microalgae, Haematococcus pluvialis and Phaeodactylum tricornutum. These microalgae were grown in appropriate optimized medium supplemented with 25 % (v/v) deuterated water. LC-MS analysis showed a maximum of 20, 25, 23, 24, and 27 % replacement of hydrogen by deuterium atoms respectively in astaxanthin, β-carotene, lutein, chlorophyll-a, and EPA. To our knowledge, this is the first report on the production of deuterated astaxanthin, chlorophyll-a and EPA by these microalgae.
AB - To understand the effect of any biomolecules in specific metabolic pathways in humans, bioavailability and for other basic understanding, stable isotopically-labelled biomolecules (preferably deuterated) is the fundamental pre-requisite. Production of deuterated biomolecules such as, astaxanthin, β-carotene, lutein, chlorophyll-a, and eicosapentaenoic acid (EPA, 20:5n-3) by metabolic tagging have been shown in commercially important microalgae, Haematococcus pluvialis and Phaeodactylum tricornutum. These microalgae were grown in appropriate optimized medium supplemented with 25 % (v/v) deuterated water. LC-MS analysis showed a maximum of 20, 25, 23, 24, and 27 % replacement of hydrogen by deuterium atoms respectively in astaxanthin, β-carotene, lutein, chlorophyll-a, and EPA. To our knowledge, this is the first report on the production of deuterated astaxanthin, chlorophyll-a and EPA by these microalgae.
KW - Deuterated astaxanthin
KW - Deuterated chlorophyll-a
KW - Deuterated eicosapentaenoic acid
KW - Deuterated lutein
KW - Deuterated β-carotene
KW - Microalgae
UR - http://www.scopus.com/inward/record.url?scp=84878947725&partnerID=8YFLogxK
U2 - 10.1007/s10529-013-1176-8
DO - 10.1007/s10529-013-1176-8
M3 - Article
C2 - 23479414
AN - SCOPUS:84878947725
SN - 0141-5492
VL - 35
SP - 1067
EP - 1072
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 7
ER -