Tagging of biomolecules with deuterated water (D2O) in commercially important microalgae

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11 Citations (Scopus)

Abstract

To understand the effect of any biomolecules in specific metabolic pathways in humans, bioavailability and for other basic understanding, stable isotopically-labelled biomolecules (preferably deuterated) is the fundamental pre-requisite. Production of deuterated biomolecules such as, astaxanthin, β-carotene, lutein, chlorophyll-a, and eicosapentaenoic acid (EPA, 20:5n-3) by metabolic tagging have been shown in commercially important microalgae, Haematococcus pluvialis and Phaeodactylum tricornutum. These microalgae were grown in appropriate optimized medium supplemented with 25 % (v/v) deuterated water. LC-MS analysis showed a maximum of 20, 25, 23, 24, and 27 % replacement of hydrogen by deuterium atoms respectively in astaxanthin, β-carotene, lutein, chlorophyll-a, and EPA. To our knowledge, this is the first report on the production of deuterated astaxanthin, chlorophyll-a and EPA by these microalgae.

Original languageEnglish
Pages (from-to)1067-1072
Number of pages6
JournalBiotechnology Letters
Volume35
Issue number7
DOIs
Publication statusPublished - Jul 2013

Keywords

  • Deuterated astaxanthin
  • Deuterated chlorophyll-a
  • Deuterated eicosapentaenoic acid
  • Deuterated lutein
  • Deuterated β-carotene
  • Microalgae

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