@inbook{93133e4a4cf84bcb8a74b6b5c1910cd3,
title = "Rapid Laboratory Diagnosis of Epizootic Hemorrhagic Disease Virus Using a Loop-Mediated Isothermal Amplification Assay",
abstract = "Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is a molecular diagnostic assay that is particularly useful for the detection of viral diseases of livestock. A major advantage of RT-LAMP is that it can be used either as a rapid field test or as a high-throughput screening tool in veterinary laboratories, with sensitivity comparable to the real-time RT-PCR assay. Unlike conventional or qPCR, RT-LAMP uses a strand displacement polymerase and a set of four to six primers that bind to several regions of the target nucleic acid. Amplification occurs without thermal cycling, and coupled with the numerous primers, RT-LAMP offers a rapid and highly specific molecular assay. In this chapter, we describe the RT-LAMP protocol for the detection of epizootic hemorrhagic disease virus (EHDV) as a low-cost, specific, and sensitive screening tool in veterinary diagnostic laboratories. We also provide guidance on how to adapt the RT-LAMP assay for rapid field testing.",
keywords = "Diagnosis, Epizootic hemorrhagic disease virus, LAMP, Loop-mediated isothermal amplification assay",
author = "Paulina Rajko-Nenow and John Flannery",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-4035-7\_12",
language = "English",
isbn = "9781071640340",
volume = "2838",
series = "Methods in Molecular Biology",
publisher = "Springer Nature",
pages = "171--183",
booktitle = "Epizootic Hemorrhagic Disease Virus:",
address = "United Kingdom",
}