TY - JOUR
T1 - Overexpression of Cu/Zn superoxide dismutase (Cu/Zn SOD) in synechococcus elongatus PCC 7942 for enhanced azo dye removal through hydrogen peroxide accumulation
AU - Mohandass, Shylajanaciyar
AU - Ragavan, Mangalalakshmi
AU - Gnanasekaran, Dineshbabu
AU - Lakshmanan, Uma
AU - Dharmar, Prabaharan
AU - Saha, Sushanta Kumar
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12
Y1 - 2021/12
N2 - Discharge of recalcitrant azo dyes to the environment poses a serious threat to environmental health. However certain microorganisms in nature have developed their survival strategies by degrading these toxic dyes. Cyanobacteria are one such prokaryotic, photosynthetic group of microorganisms that degrade various xenobiotic compounds, due to their capability to produce various reactive oxygen species (ROS), and particularly the hydrogen peroxide (H2 O2) when released in their milieu. The accumulation of H2 O2 is the result of the dismutation of superoxide radicals by the enzyme superoxide dismutase (SOD). In this study, we have genetically modified the cyanobacterium Synechococcus elongatus PCC 7942 by integrating Cu/Zn SOD gene (sodC) from Synechococcus sp. PCC 9311 to its neutral site through homologous recombination. The overexpression of sodC in the derivative strain was driven using a strong constitutive promoter of the psbA gene. The derivative strain resulted in constitutive production of sodC, which was induced further during dye-treated growth. The genetically engineered Synechococcus elongatus PCC 7942 (MS-sodC+) over-accumulated H2 O2 during azo dye treatment with a higher dye removal rate than the wild-type strain (WS-sodC−). Therefore, enhanced H2 O2 accumulation through SODs overexpression in cyanobacteria may serve as a valuable bioremediation tool.
AB - Discharge of recalcitrant azo dyes to the environment poses a serious threat to environmental health. However certain microorganisms in nature have developed their survival strategies by degrading these toxic dyes. Cyanobacteria are one such prokaryotic, photosynthetic group of microorganisms that degrade various xenobiotic compounds, due to their capability to produce various reactive oxygen species (ROS), and particularly the hydrogen peroxide (H2 O2) when released in their milieu. The accumulation of H2 O2 is the result of the dismutation of superoxide radicals by the enzyme superoxide dismutase (SOD). In this study, we have genetically modified the cyanobacterium Synechococcus elongatus PCC 7942 by integrating Cu/Zn SOD gene (sodC) from Synechococcus sp. PCC 9311 to its neutral site through homologous recombination. The overexpression of sodC in the derivative strain was driven using a strong constitutive promoter of the psbA gene. The derivative strain resulted in constitutive production of sodC, which was induced further during dye-treated growth. The genetically engineered Synechococcus elongatus PCC 7942 (MS-sodC+) over-accumulated H2 O2 during azo dye treatment with a higher dye removal rate than the wild-type strain (WS-sodC−). Therefore, enhanced H2 O2 accumulation through SODs overexpression in cyanobacteria may serve as a valuable bioremediation tool.
KW - Copper/zinc superoxide dismutase (Cu/Zn SOD)
KW - Cyanobacteria
KW - Hydrogen peroxide (H O)
KW - Superoxide dismutase (SOD)
KW - Synechococcus elongatus PCC 7942
UR - http://www.scopus.com/inward/record.url?scp=85121600562&partnerID=8YFLogxK
U2 - 10.3390/biology10121313
DO - 10.3390/biology10121313
M3 - Article
AN - SCOPUS:85121600562
SN - 2079-7737
VL - 10
JO - Biology
JF - Biology
IS - 12
M1 - 1313
ER -
