TY - GEN
T1 - Monitoring of extracellular matrix protein conformations in the presence of biomimetic bone tissue regeneration scaffolds
AU - Rodriguez Barroso, L.
AU - Lanzagorta Garcia, E.
AU - Azaman, F. A.
AU - Devine, D. M.
AU - Lynch, M.
AU - Huerta, M.
AU - Fournet, M. B.
N1 - Publisher Copyright:
© 2020 Trans Tech Publications Ltd, Switzerland.
PY - 2020
Y1 - 2020
N2 - Tissue scaffolds can be designed to mimic the native extracellular matrix (ECM), making them attractive for the development for a range of regenerative medicine applications. The macromolecules present in the ECM are critical for the provision of structural support to surrounding cells and signalling cues for the modulation of diverse processes including cell migration, proliferation and healing activation. Here, conformational and transitional behaviour of the ubiquitous ECM protein, fibronectin (Fn), in the presence of bone tissue regeneration scaffolds and living C2C12 myoblast cells is reported. Spectral monitoring of Fn functionalised high plasmonic resonance responsive gold-edge-coated triangular silver nanoplates (AuTSNP) is used to distinguish between compact and extended fibronectin conformations. Large spectral red shifts of ~20 to ~57 nm indicate Fn unfolding and fibril formation on incubation with C2C12 cells. The label-free nature, excellent sensitivity and straightforward application of the AuTSNP within cellular environments presents them as a powerful new tool to signature protein conformational activity in living cells and monitor essential protein activity for the assisted development of improved tissue scaffolds promoting enhanced tissue repair.
AB - Tissue scaffolds can be designed to mimic the native extracellular matrix (ECM), making them attractive for the development for a range of regenerative medicine applications. The macromolecules present in the ECM are critical for the provision of structural support to surrounding cells and signalling cues for the modulation of diverse processes including cell migration, proliferation and healing activation. Here, conformational and transitional behaviour of the ubiquitous ECM protein, fibronectin (Fn), in the presence of bone tissue regeneration scaffolds and living C2C12 myoblast cells is reported. Spectral monitoring of Fn functionalised high plasmonic resonance responsive gold-edge-coated triangular silver nanoplates (AuTSNP) is used to distinguish between compact and extended fibronectin conformations. Large spectral red shifts of ~20 to ~57 nm indicate Fn unfolding and fibril formation on incubation with C2C12 cells. The label-free nature, excellent sensitivity and straightforward application of the AuTSNP within cellular environments presents them as a powerful new tool to signature protein conformational activity in living cells and monitor essential protein activity for the assisted development of improved tissue scaffolds promoting enhanced tissue repair.
KW - Extracellular matrix
KW - Fibronectin
KW - Local surface plasmon resonance
KW - Tissue scaffolds
KW - Triangular silver nanoplates
UR - http://www.scopus.com/inward/record.url?scp=85093076674&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/KEM.865.43
DO - 10.4028/www.scientific.net/KEM.865.43
M3 - Conference contribution
AN - SCOPUS:85093076674
SN - 9783035715774
T3 - Key Engineering Materials
SP - 43
EP - 47
BT - Materials Science and Smart Materials
A2 - Ramadan, Mohamad
A2 - Ramadan, Mohamad
A2 - Olabi, Abdul Ghani
PB - Trans Tech Publications Ltd
T2 - 6th International Conference on Material Science and Smart Materials, MSSM 2019
Y2 - 23 July 2019 through 26 July 2019
ER -