Lysostaphin-Functionalized Waterborne Polyurethane/Polydopamine Coatings Effective against S. Aureus Biofilms

Buket Alkan Taş, Ekin Berksun, Cüneyt Erdinç Taş, Serkan Ünal, Hayriye Ünal

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    Hybrid waterborne polyurethane/polydopamine (WPU/PDA) matrix, showing the adhesive properties of PDA in its entirety, was utilized for the immobilization of lysostaphin (Lys), an important anti-staphylococcal agent, to obtain highly effective antibacterial and antibiofilm surface coatings. WPU/PDA matrix prepared by the encapsulation of WPU particles with PDA in aqueous dispersion was applied as coatings on substrates, and the facile incubation of the WPU/PDA-coated surfaces with Lys in aqueous solution resulted in WPU/PDA/Lys coatings that contained immobilized Lys on the surface. WPU/PDA/Lys coatings showed strong anti-Staphylococcus aureus activity with a 4 log reduction in the number of cells. Furthermore, WPU/PDA/Lys coatings were demonstrated to be durable without any enzyme leakage, and their antibacterial activity was preserved for at least 30 days and over multiple exposures to bacteria. WPU/PDA/Lys coatings presented significant antibiofilm activity against S. aureus with a 3.5 log reduction in the number of surface-attached bacteria. WPU/PDA/Lys coatings that are easy-to-apply to almost any surface, non-toxic, and environmentally friendly provide promising antibacterial and antibiofilm surfaces that are effective against S. aureus.

    Original languageEnglish
    Pages (from-to)4298-4305
    Number of pages8
    JournalACS Applied Polymer Materials
    Volume4
    Issue number6
    DOIs
    Publication statusPublished - 10 Jun 2022

    Keywords

    • antibacterial coating
    • antibiofilm coating
    • lysostaphin
    • polydopamine
    • waterborne polyurethane (WPU)

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