TY - JOUR
T1 - In vitro fibroblast and pre-osteoblastic cellular responses on laser surface modified Ti-6Al-4V
AU - Chikarakara, Evans
AU - Fitzpatrick, Patricia
AU - Moore, Eric
AU - Levingstone, Tanya
AU - Grehan, Laura
AU - Higginbotham, Clement
AU - Vázquez, Mercedes
AU - Bagga, Komal
AU - Naher, Sumsun
AU - Brabazon, Dermot
N1 - Publisher Copyright:
© 2015 IOP Publishing Ltd.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - The success of any implant, dental or orthopaedic, is driven by the interaction of implant material with the surrounding tissue. In this context, the nature of the implant surface plays a direct role in determining the long term stability as physico-chemical properties of the surface affect cellular attachment, expression of proteins, and finally osseointegration. Thus to enhance the degree of integration of the implant into the host tissue, various surface modification techniques are employed. In this work, laser surface melting of titanium alloy Ti-6Al-4V was carried out using a CO2 laser with an argon gas atmosphere. Investigations were carried out to study the influence of laser surface modification on the biocompatibility of Ti-6Al-4V alloy implant material. Surface roughness, microhardness, and phase development were recorded. Initial knowledge of these effects on biocompatibility was gained from examination of the response of fibroblast cell lines, which was followed by examination of the response of osteoblast cell lines which is relevant to the applications of this material in bone repair. Biocompatibility with these cell lines was analysed via Resazurin cell viability assay, DNA cell attachment assay, and alamarBlue metabolic activity assay. Laser treated surfaces were found to preferentially promote cell attachment, higher levels of proliferation, and enhanced bioactivity when compared to untreated control samples. These results demonstrate the tremendous potential of this laser surface melting treatment to significantly improve the biocompatibility of titanium implants in vivo.
AB - The success of any implant, dental or orthopaedic, is driven by the interaction of implant material with the surrounding tissue. In this context, the nature of the implant surface plays a direct role in determining the long term stability as physico-chemical properties of the surface affect cellular attachment, expression of proteins, and finally osseointegration. Thus to enhance the degree of integration of the implant into the host tissue, various surface modification techniques are employed. In this work, laser surface melting of titanium alloy Ti-6Al-4V was carried out using a CO2 laser with an argon gas atmosphere. Investigations were carried out to study the influence of laser surface modification on the biocompatibility of Ti-6Al-4V alloy implant material. Surface roughness, microhardness, and phase development were recorded. Initial knowledge of these effects on biocompatibility was gained from examination of the response of fibroblast cell lines, which was followed by examination of the response of osteoblast cell lines which is relevant to the applications of this material in bone repair. Biocompatibility with these cell lines was analysed via Resazurin cell viability assay, DNA cell attachment assay, and alamarBlue metabolic activity assay. Laser treated surfaces were found to preferentially promote cell attachment, higher levels of proliferation, and enhanced bioactivity when compared to untreated control samples. These results demonstrate the tremendous potential of this laser surface melting treatment to significantly improve the biocompatibility of titanium implants in vivo.
KW - beta-phase titanium
KW - biocompatibility
KW - cell attachment
KW - cell proliferation
KW - fibroblast cells
KW - laser surface melting
KW - pre-ostepblastic cells
UR - http://www.scopus.com/inward/record.url?scp=84924301224&partnerID=8YFLogxK
U2 - 10.1088/1748-6041/10/1/015007
DO - 10.1088/1748-6041/10/1/015007
M3 - Article
C2 - 25546881
AN - SCOPUS:84924301224
SN - 1748-6041
VL - 10
JO - Biomedical Materials (Bristol)
JF - Biomedical Materials (Bristol)
IS - 1
M1 - 015007
ER -