TY - JOUR
T1 - Electrospinning Recombinant Spider Silk Fibroin-Reinforced PLGA Membranes
T2 - A Biocompatible Scaffold for Wound Healing Applications
AU - Chen, Yuanyuan
AU - Murphy, Emma J.
AU - Cao, Zhi
AU - Buckley, Ciara
AU - Cortese, Yvonne
AU - Chee, Bor Shin
AU - Scheibel, Thomas
N1 - Publisher Copyright:
© 2024 American Chemical Society.
PY - 2024
Y1 - 2024
N2 - Polylactide-polyglycolide (PLGA) is one of the most attractive polymeric biomaterials used to fabricate medical devices for drug delivery and tissue engineering applications. Nevertheless, the utilization of PLGA in load-bearing applications is restricted due to its inadequate mechanical properties. This study examines the potential of recombinant silk fibroin (eADF4), a readily producible biomaterial, as a reinforcing agent for PLGA. The PLGA/eADF4 composite membranes were developed by using the process of electrospinning. The spinnability of the electrospinning solutions and the physicochemical, mechanical, and thermal properties of the composite membranes were characterized. The addition of eADF4 increased the viscosity of the electrospinning solutions and enhanced both the mechanical characteristics and the thermal stability of the composites. This study demonstrates that PLGA membranes reinforced with recombinant spider silk fibroin are noncytotoxic, significantly enhance cell migration and wound closure, and do not trigger an inflammatory response, making them ideal candidates for advanced wound healing applications.
AB - Polylactide-polyglycolide (PLGA) is one of the most attractive polymeric biomaterials used to fabricate medical devices for drug delivery and tissue engineering applications. Nevertheless, the utilization of PLGA in load-bearing applications is restricted due to its inadequate mechanical properties. This study examines the potential of recombinant silk fibroin (eADF4), a readily producible biomaterial, as a reinforcing agent for PLGA. The PLGA/eADF4 composite membranes were developed by using the process of electrospinning. The spinnability of the electrospinning solutions and the physicochemical, mechanical, and thermal properties of the composite membranes were characterized. The addition of eADF4 increased the viscosity of the electrospinning solutions and enhanced both the mechanical characteristics and the thermal stability of the composites. This study demonstrates that PLGA membranes reinforced with recombinant spider silk fibroin are noncytotoxic, significantly enhance cell migration and wound closure, and do not trigger an inflammatory response, making them ideal candidates for advanced wound healing applications.
KW - electrospinning
KW - mechanical properties
KW - polylactide−polyglycolide
KW - recombinant spider silk fibroin
KW - reinforcement
KW - wound healing
UR - http://www.scopus.com/inward/record.url?scp=85207284388&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.4c01605
DO - 10.1021/acsbiomaterials.4c01605
M3 - Article
C2 - 39435963
AN - SCOPUS:85207284388
SN - 2373-9878
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
ER -