TY - JOUR
T1 - Effect of macro- and micro-nutrient limitation on superoxide dismutase activities and carotenoid levels in microalga Dunaliella salina CCAP 19/18
AU - Saha, Sushanta Kumar
AU - Moane, Siobhan
AU - Murray, Patrick
PY - 2013/11
Y1 - 2013/11
N2 - The aim of this study was to assess the effects of sixteen stress conditions on total carotenoid production and the response of antioxidative enzyme superoxide dismutase (SOD) in microalga Dunaliella salina. Of the stress conditions tested, high-light illumination (240μmolphotonsm-2s-1) in combination with nitrogen depletion were the conditions associated with maximum carotenoid production and which induced Fe-SOD and retained the specific Mn-SOD isoform. Removal of the micronutrients manganese (Mn), Zinc (Zn) and Iron (Fe) as well as nitrogen from the medium enhanced carotenoid production on day 5, while the removal of nitrogen and Mn from the growth medium drastically affected carotenoid production at all time-points. The differential response of SODs influences the levels of carotenoid biosynthesis as chronic molecular defence strategies of D. salina.
AB - The aim of this study was to assess the effects of sixteen stress conditions on total carotenoid production and the response of antioxidative enzyme superoxide dismutase (SOD) in microalga Dunaliella salina. Of the stress conditions tested, high-light illumination (240μmolphotonsm-2s-1) in combination with nitrogen depletion were the conditions associated with maximum carotenoid production and which induced Fe-SOD and retained the specific Mn-SOD isoform. Removal of the micronutrients manganese (Mn), Zinc (Zn) and Iron (Fe) as well as nitrogen from the medium enhanced carotenoid production on day 5, while the removal of nitrogen and Mn from the growth medium drastically affected carotenoid production at all time-points. The differential response of SODs influences the levels of carotenoid biosynthesis as chronic molecular defence strategies of D. salina.
KW - Antioxidative enzyme
KW - Carotenoid
KW - Dunaliella salina
KW - Micronutrient limitation
KW - Superoxide dismutase
UR - http://www.scopus.com/inward/record.url?scp=84882959491&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2013.08.022
DO - 10.1016/j.biortech.2013.08.022
M3 - Article
C2 - 23981270
AN - SCOPUS:84882959491
SN - 0960-8524
VL - 147
SP - 23
EP - 28
JO - Bioresource Technology
JF - Bioresource Technology
ER -