Cryopreservation of vegetative thalli of Ulva species

Clara Simon, Antoine Fort, Ronan Sulpice

Research output: Contribution to journalArticlepeer-review

Abstract

Sea lettuce (Ulva) is a genus of green macroalgae present along all the coasts of the world's oceans. It represents about 100 species with diverse habitats. Inter- and intra-species natural variation is very large, both in terms of growth characteristics and biomass biochemical composition. As a result, Ulva biomass has a wide range of applications and strain selection can achieve significant increases in yield(s). Establishing solid, long term and cost-effective methodologies for the conservation of Ulva genetic diversity is then required to safeguard and reuse selected strains. Here, we report a cryopreservation-based protocol for the long-term preservation of foliose Ulva strains. Strains from seven different Ulva species were cryopreserved for 15 and/or 120 days in liquid nitrogen, and of the 3 replicates cryopreserved, at least one survived, allowing us to successfully recover all strains. On average, among all specimen cryo-preserved, 82% of them survived and grew post cryo-preservation.

Original languageEnglish
Pages (from-to)3011-3016
Number of pages6
JournalJournal of Applied Phycology
Volume36
Issue number5
DOIs
Publication statusPublished - Oct 2024

Keywords

  • Aquaculture
  • Biobanking
  • Chlorophyceae
  • Cryopreservation
  • Ulva

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