Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

Deborah M. Waters, Patrick G. Murray, Yuta Miki, Angel T. Martínez, Maria G. Tuohy, Craig B. Faulds

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.

Original languageEnglish
Pages (from-to)3759-3762
Number of pages4
JournalApplied and Environmental Microbiology
Volume78
Issue number10
DOIs
Publication statusPublished - May 2012

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