TY - JOUR
T1 - Cloning, heterologous expression, and characterization of the xylitol and L-arabitol dehydrogenase genes, Texdh and telad, from the thermophilic fungus Talaromyces emersonii
AU - Fernandes, Sara
AU - Tuohy, Maria G.
AU - Murray, Patrick G.
N1 - Funding Information:
Acknowledgments This work was supported by an Irish government Department of Agriculture, Food and Fisheries award (DAFF RSF-05-225) under the Research stimulus Fund Programme, NDP 2000-06 and 2007-13, and Enterprise Ireland Proof of Concept award to MGT.
PY - 2010/6
Y1 - 2010/6
N2 - The genes encoding xylitol dehydrogenase (Texdh) and L-arabitol dehydrogenase (Telad) are involved in the fungal pentose pathway and were isolated from the thermophilic fungus Talaromyces emersonii, expressed in Escherichia coli, and the products purified to homogeneity. TeXDH showed activity toward xylitol and D-sorbitol. TeLAD was active with L-arabitol, xylitol, and D-sorbitol. Phylogenetic analysis showed TeLAD has evolved from D-sorbitol dehydrogenase as a result of environmental adaptation. Substrate specificity studies indicate that TeXDH is likely to have evolved from the more broadly acting TeLAD. Texdh and Telad expression was inducible by the same carbon sources responsible for induction of genes involved in biomass degradation, suggesting for the first time a coordinated regulatory control mechanism for expression of genes encoding extracellular hydrolases and intracellular metabolic genes in the pentose utilization pathways of T. emersonii. These data also suggest that TeXDH and TeLAD may be valuable in the production of xylitol, L-arabitol, and ethanol from renewable resources rich in pentose sugars.
AB - The genes encoding xylitol dehydrogenase (Texdh) and L-arabitol dehydrogenase (Telad) are involved in the fungal pentose pathway and were isolated from the thermophilic fungus Talaromyces emersonii, expressed in Escherichia coli, and the products purified to homogeneity. TeXDH showed activity toward xylitol and D-sorbitol. TeLAD was active with L-arabitol, xylitol, and D-sorbitol. Phylogenetic analysis showed TeLAD has evolved from D-sorbitol dehydrogenase as a result of environmental adaptation. Substrate specificity studies indicate that TeXDH is likely to have evolved from the more broadly acting TeLAD. Texdh and Telad expression was inducible by the same carbon sources responsible for induction of genes involved in biomass degradation, suggesting for the first time a coordinated regulatory control mechanism for expression of genes encoding extracellular hydrolases and intracellular metabolic genes in the pentose utilization pathways of T. emersonii. These data also suggest that TeXDH and TeLAD may be valuable in the production of xylitol, L-arabitol, and ethanol from renewable resources rich in pentose sugars.
KW - L-arabitol dehydrogenase
KW - Talaromyces emersonii
KW - Xylitol dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=77953132070&partnerID=8YFLogxK
U2 - 10.1007/s10528-010-9332-5
DO - 10.1007/s10528-010-9332-5
M3 - Article
C2 - 20111899
AN - SCOPUS:77953132070
SN - 0006-2928
VL - 48
SP - 480
EP - 495
JO - Biochemical Genetics
JF - Biochemical Genetics
IS - 5-6
ER -