Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumour Cells

Anne M. Friel, John Crown, Lorraine O’Driscoll

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Current literature provides significant evidence to support the concept that there are limited subpopulations of cells within a solid tumour that have increased tumour-initiating potential relative to the total tumour population. Such tumour-initiating cells have been identified in leukaemia and in a variety of solid tumours using different combinations of cell surface markers, suggesting that a tumour-initiating cell heterogeneity exists for each specific tumour. These studies have been extended to endometrial cancer; and herein we present several experimental approaches, both in vitro and in vivo, that can be used to determine whether such populations exist, and if so, to characterize them. These methods are adaptable to the investigation of tumour-initiating cells from other tumour types.

Original languageEnglish
Title of host publicationGene Expression Profiling
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages55-75
Number of pages21
ISBN (Print)9781617792885
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Molecular Biology
Volume784
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Bone marrow
  • Cancer stem cell
  • Circulating tumour cells
  • Endometrial cancer
  • Peripheral blood mononuclear cells
  • Surface markers
  • Tumour-initiating cell
  • Xenograft model

Fingerprint

Dive into the research topics of 'Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumour Cells'. Together they form a unique fingerprint.

Cite this